Elucidating altered transcriptional programs
Genome editing has enabled broad advances and novel approaches in studies of gene function and structure; now, emerging methods aim to precisely engineer post-transcriptional processes.
Developing precise, efficient molecular tools to alter the transcriptome holds great promise for biotechnology and synthetic biology applications.
Bassel GW, Fung P, Chow TF, Foong JA, Provart NJ, Cutler SR Plant Physiol.
2008 May;147(1):143-55 PMID: 18359847 The transition from seed to seedling is mediated by germination, a complex process that starts with imbibition and completes with radicle emergence.
Different approaches have been employed for targeted degradation of RNA species in eukaryotes, but they lack programmability and versatility, thereby limiting their utility for diverse applications.
The CRISPR/Cas9 system has been harnessed for genome editing in many eukaryotic species and, using a catalytically inactive Cas9 variant, the CRISPR/d Cas9 system has been repurposed for transcriptional regulation.
In project 2 we will elucidate the biochemical mechanism.
Such dependence on SE-driven transcription for proliferation and survival offers an Achilles heel for the therapeutic targeting of cancer cells.
Furthermore, JARID1C contributed to drug resistance. Thus in project 1, we will address mechanistically how JARID1C is activated, and whether disrupting JARID1C would defeat the drug resistance.
We have identified that VHL protein regulates the protein stability of the oncogene EGFR.
In this study, three mechanistically distinct small molecules that inhibit Arabidopsis seed germination (methotrexate, 2, 4-dinitrophenol, and cycloheximide) were identified using a small-molecule screen and used to probe the germination transcriptome.
Germination-responsive transcripts were defined as those with significantly altered transcript abundance across all inhibitory treatments with respect to control germinating seeds, using data from ATH1 microarrays.